Glyceraldehyde-3-Phosphate Dehydrogenase(NADP) from Sinapis alba L.. NAD(P)-Induced Conformation Changes of the Enzyme
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منابع مشابه
Liposome-interaction induced conformation changes of glyceraldehyde-3-phosphate dehydrogenase.
Tryptophanyl emission spectra of rabbit muscle glyceraldehyde-3-phosphate dehydrogenase (G3PDH) were measured after the addition of liposomes prepared of natural phospholipids: phosphatidylinositols (PI), phosphatidylserines (PS) and phosphatidylcholines (PC). The measurings were made for various molar lipid/protein ratios (100-1000). A decrease in the enzyme fluorescence intensity and a "red" ...
متن کاملGlyceraldehyde 3-Phosphate Dehydrogenases and Glyoxylate Reductase: II. Far Red Light-Dependent Development of Glyceraldehyde 3-Phosphate Dehydrogenase Isozyme Activities in Sinapis Alba Cotyledons.
Ammonium sulfate chromatography has been employed to separate glyceraldehyde 3-phosphate dehydrogenases (GPD) of Sinapis alba cotyledons of various developmental stages. Cotyledons of dark-grown seedlings possess one major NAD-specific enzyme designated NAD-GPD I. Irradiation with continuous far red light leads to a strong increase in NADP-GPD activity and to the formation of a second NAD activ...
متن کاملProkaryotic features of a nucleus-encoded enzyme. cDNA sequences for chloroplast and cytosolic glyceraldehyde-3-phosphate dehydrogenases from mustard (Sinapis alba).
Two cDNA clones, encoding cytosolic and chloroplast glyceraldehyde-3-phosphate dehydrogenases (GAPDH) from mustard (Sinapis alba), have been identified and sequenced. Comparison of the deduced amino acid sequences with one another and with the GAPDH sequences from animals, yeast and bacteria demonstrates that nucleus-encoded subunit A of chloroplast GAPDH is distinct from its cytosolic counterp...
متن کاملActivity of Glyceraldehyde-3-Phosphate Dehydrogenase-NADP in Developing Leaves of Light-Grown Dianthus chinensis L.
Quantitative histochemical methods were used to measure glyceraldehyde-3-phosphate dehydrogenase-NADP activity in developing and mature leaves of Dianthus chinensis L. The activity values were compared on the basis of illumination of developing leaves, the maturity of stomata, and the activity of enzymes concerned with carbohydrate oxidation. Activity on a kilogram dry weight per hour basis inc...
متن کاملGlyceraldehyde 3-Phosphate Dehydrogenases and Glyoxylate Reductase: I. Their Regulation Under Continuous Red and Far Red Light in the Cotyledons of Sinapis alba L.
The development of NADP- and NAD-dependent glyceraldehyde 3-phosphate dehydrogenase and NADH-specific glyoxylate reductase was followed in Sinapis alba cotyledons grown in the dark or under continuous red and far red light. All three enzyme activities are promoted by light, continuous far red light being more than twice as effective as continuous red light. The activities of the NADP-glyceralde...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1978
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1978.tb11995.x